Does vitamin D have an effect on osseointegration of dental implants? A systematic review
International Journal of Implant Dentistry volume 8, Article number: 16 (2022)
The aim of this study was to systematically review the available evidence to evaluate the efficacy of vitamin D supplementation or vitamin D depletion on the osseointegration of implants in animals and humans.
The focus questions addressed were “Do vitamin D deficient subjects treated with (dental) implants have an inferior osseointegration than subjects with adequate serum vitamin D level?” and “Do vitamin D supplemented subjects treated with (dental) implants have a superior osseointegration than subjects with adequate serum vitamin D level?” Humans and animals were considered as subjects in this study. Databases were searched from 1969 up to and including March 2021 using different combination of the following terms: “implant”, “bone to implant contact”, “vitamin D” and “osseointegration”. Letters to the editor, historic reviews, commentaries and articles published in languages other than English and German were excluded. The pattern of the present systematic review was customize to primarily summarize the pertinent data.
Thirteen experimental studies with animals as subject, two clinical studies and three case reports, with humans as subjects, were included. The amount of inserted titanium implants ranged between 24 and 1740. Results from three animal studies showed that vitamin D deficiency has a negative effect on new bone formation and/or bone to implant contact (BIC). Eight animal studies showed that vitamin D supplementation has a enhancing effect on BIC and/or new bone formation around implants. Furthermore, enhancing the impact of vitamin D supplementation on the osseointegration of implants in subjects with diabetes mellitus, osteoporosis and chronic kidney disease (CKD) were assessed. Studies and case reports involving human subjects showed that patients with a low serum vitamin D level have a higher tendency to exhibit an early dental implant failure. When supplemented with vitamin D the osseointegration was successful in the case reports and a beneficial impact on the changes in the bone level during the osseointegration were determined.
Vitamin D deficiency seems to have a negative effect on the osseointegration of implants in animals. The supplementation of vitamin D appears to improve the osseointegration in animals with systemic diseases, such as vitamin D deficiency, diabetes mellitus, osteoporosis, and CKD. Slight evidence supports the hypothesis that humans similarly benefit from vitamin D supplementation in terms of osseointegration. Further investigation is required to maintain these assumptions.
Successful osseointegration is one of the key criteria for a prosperous dental implant therapy which is achieved by a functional ankylosis. The foreign material and vital bone grow together as a functional unit which is characterized as the initial newly created bone to implant contact (BIC) [1, 2]. New bone formation can be both enhanced  or decelerated [4, 5] due to vitamin D, depending on its level. Vitamin D deficiency is associated with a variety of diseases, such as parodontitis [6, 7], early tooth loss , a catabolic metabolism, osteoporotic fractures  and compromised fracture healing [9, 10]. Vitamin D is a steroid hormone which can be synthesized in the skin when sun irradiation is sufficient (290–315 nm) and successfully converted in the liver and kidneys . Sufficient sun irradiation is only available in months from April until September northern 40° N latitude, which is northern from Madrid . Therefore, most humans living in Europe, northern Asia and North America are not adequately supplied with vitamin D throughout the year from sunlight alone. Vitamin D is additionally contained in nourishment, such as oily fish, liver (beef), eggs, milk, cheese soy and mushrooms [13, 14]. It plays an important role in the mineral homeostasis by stimulating intestinal absorption of calcium and phosphate . It also regulates the bone metabolism and bone mineralization by activating osteoclasts and osteoblasts [5, 16, 17].
Vitamin D is available in different sources and, therefore, has different versions. Ergocalciferol (Vitamin D2) is the versions contained in plants and Cholecalciferol (Vitamin D3) in animals and humans [18, 19] (Fig. 1). There are also active and inactive versions of vitamin D depending on the way of hydroxylation. For laboratory measurements 25 (OH) vitamin D status is determined, since it is a metabolite with a high concentration and a relatively long half-life. The active version, however, is 1,25 (OH) vitamin D. Since the laboratory measurement detects only the level of 25 (OH) vitamin D and solely in the bloodstream it is not a perfectly accurate method to measure all disposable vitamin D inside the body. However, it is a good measurement of the available vitamin D in the bloodstream and the established marker of vitamin D status [19, 20]. Furthermore, multiple laboratory measurements are available to determine the concentration of other vitamin D metabolites, but none of them were part of studies included in this systematic review.
Schulze-Späte et al.  assessed the biological effect of systemic vitamin D supplementation on bone cells. This randomized, double-blind, placebo-controlled clinical investigation showed that patients supplemented with vitamin D demonstrate a higher osteoclast activity compared with control. Both positive [22,23,24,25,26,27] and negative  effects of vitamin D supplementation on BIC have been mentioned in the previous literature. Liu et al.  and Zhou et al.  for instance showed a significant enhanced bone volume and osseointegration compared with control. However, Naito et al.  and Akhavan et al.  did not show a significant effect of vitamin D supplementation in terms of increased BIC. Concerning vitamin D deficiency, all studies [23, 31, 32] showed a significant decreasing BIC or new bone formation compared to control or vitamin D supplemented rats. There seems to be a disagreement over the efficacy of vitamin D supplementation concerning the augmenting BIC. Hence the aim of this study was to systematically review the available evidence to evaluate the efficacy of vitamin D supplementation or vitamin D depletion on the osseointegration of implants.
Materials and methods
Based on the preferred reporting items for systematic reviews and meta-analysis (PRISMA) guidelines, two particular questions were created according to the participants, intervention, control, outcomes (PICO) principle (Fig. 2). The constructed Questions were:
Do vitamin D deficient subjects treated with (dental) implants have an inferior osseointegration than subjects with adequate serum vitamin D level and.
do vitamin D supplemented subjects treated with (dental) implants have a superior osseointegration than subjects with adequate serum vitamin D level?
(P) Participants: it was crucial for subjects (animals or humans) to have undergone implant treatment.
(I) Types of interventions: the intervention of interest was the effect of vitamin D deficiency on osseointegration.
(C) Control intervention: osseointegration with adequate vitamin D serum level.
(O) Outcome measures: bone to implant contact (BIC), new bone formation (NBF) and/or Implant resistance, bone volume/tissue volume (BV/TV) around the implants with and without vitamin D deficiency.
(P) Participants: it was crucial for subjects (animals or humans) to have undergone implant treatment.
(I) Types of intervention: the intervention of interest was the effect of vitamin D supplementation on osseointegration.
(C) Control intervention: osseointegration with adequate vitamin D serum level.
(O) Outcome measures: bone to implant contact (BIC), new bone formation (NBF) and/or Implant resistance, bone volume/tissue volume (BV/TV) around the implants with and without vitamin D supplementation.
The eligibility criteria were as follows: (a) original studies or case reports (clinical and experimental), (b) inclusion of a control group (osseointegration around implants without vitamin D supplementation/deficiency) (c) intervention: effect of vitamin D supplementation/deficiency on osseointegration (d) conservative non-intervention studies and (e) studies published in English and German language. Letters to the editor, historic reviews and commentaries were excluded.
Literature search protocol
PubMed/Medline (National library of Medicine, Washington, DC, USA), Cochane library and Google-Scholar databases was searched from 1969 to March 2021 using different combination of the following terms: “implant”, “bone to implant contact”, “vitamin D” and “osseointegration”. Titles and abstracts of studies identified using the above-described protocol were screened by the author (JW). Full texts of studies judged by title and abstract to be relevant were read and independently evaluated for the stated eligibility criteria. Reference lists of potentially relevant original and review articles were hand searched to identify any studies that could have remained unidentified in the previous step. The pattern of the present systematic review was customized to mainly summarize the relevant data. The initial search yielded 60 studies. Forty two studies which did not fulfill the eligibility criteria were excluded. In total, 18 experimental [22,23,24,25,26,27,28,29,30,31,32,33,34,35] (Table 1) and non-intervention clinical studies  and case reports [37,38,39] were included and processed for data extraction (Table 2).
Results of studies carried out with animals
The majority [22,23,24,25,26,27,28,29,30,31,32,33,34] of the studies were performed with animals. Few studies [35, 36] and case reports [37,38,39] were performed with humans, respectively. One study  was performed with mice. Eight studies [22, 23, 25, 30,31,32,33,34] were performed with rats, of which six studies involved only one sex. Each sex was included three times separately, male [22, 32, 33] and female [23, 25, 34] rats. In two [30, 31] studies the sex of the used rats was not reported. Three studies [27,28,29] included rabbits of which one  was performed with females only. One study  was performed with dogs. In almost all animal studies [22,23,24,25,26,27,28,29,30,31, 33, 34] Ti implants were used, one study  was performed with Demineralized Allogeneic Bone Matrix (DABM) as implant material. The number of inserted implants ranged between 24 and 168. The number of placed implants was not reported in two studies [22, 29]. Dimensions (diameter × length in millimeters) ranged between 1 × 2, 6.25 × 2 and 1.4 × 23. Implants were placed in tibia, femur and jaw in seven [23, 25, 27,28,29,30, 33], four [22, 24, 31, 34] and one study , respectively. In the study by Vandersteenhoven et al.  the region of the implantation was subcutaneously in abdominal sites. The follow-up period of the studies ranged between 1 and 8 weeks. In four studies [26,27,28,29] vitamin D coated and non-coated implants were evaluated on their impact on osseointegration. Moreover, the effect of vitamin D supplementation on osseointegration was assessed in ovariectomized rats [23, 25, 34], diabetic rats [22, 30], nephrectomized mice  and vitamin D deficient rats [23, 31, 32].
Assessment of osseointegration
In eleven studies [22,23,24,25,26,27, 29,30,31,32, 34] histological analysis was used to assess the osseointegration. In six studies [22, 25, 28, 31, 33, 34] the implant resistance was assessed due to biomechanical tests, the new bone formation and strength of the newly formed bone around the implant were measured. In three studies [22, 25, 33] the new bone formation around implants was assessed using a three-dimensional (3D) microscopic computerized tomography (micro-CT), two studies [24, 26] used conventional two-dimensional (2D) radiography to verify implant osseointegration and to assess changes in post-surgical crestal bone levels, respectively. In seven studies [22,23,24,25,26,27, 29, 32] histomorphometry was used to assess the osseointegration around the implants and scanning electron microscope (SEM) was used in two studies [31, 34]. Wound fluid analysis was performed in the study by Satué et al.  to assess the cellular activity while osseointegration. Serum vitamin D level have been measured in one study .
The results of three animal studies [23, 31, 32] showed that vitamin D deficiency declined new bone formation and/or BIC around implants. Eight studies [22,23,24,25,26,27, 32, 34] involving animals provided adequately with vitamin D showed that vitamin D supplementation enhanced new bone formation and/or BIC around implants. Four studies reported no statistically significant differences in BIC and/or new bone formation around vitamin D coated implants [28, 29] or systemic vitamin D supplementation [30, 33]. Diabetic rats had an improved osseointegration when treated with insulin . Additional vitamin D supplementation enhanced the new bone formation around implants in diabetic rats , while vitamin D supplementation solely had no significant statistical effect . A different study  reported significant enhanced new bone formation and BIC in nephrectomized mice when supplemented with vitamin D. Significant improvement in implant resistance and bone area density was achieved in ovariectomized rats after treatment with a combination of vitamin D and Alendronate  (Fig. 3).
Results of clinical studies
Humans were subjects in two studies [35, 36] and three case reports [37,38,39]. The case reports were developed with male patients only. In all studies and case reports [35,36,37,38,39] humans had undergone dental implant treatment with Ti [35,36,37,38] or TiZr  implants. The number of inserted implants ranged between 4 and 1740. Dimensions (diameter × length in millimeters) ranged between 3.3 mm × 8 mm, 4.1 mm × 12 mm and 4.3 mm × 7 mm. The follow-up period of the studies ranged between 12 weeks and 7 months. One study  evaluated the effect of vitamin D supplementation on changes in the bone level at implant site during the process of osseointegration. A different study  aimed to discover the ratio of early dental implant failures (EDIF = i.e., failure that occurs within 4 months after placement, before the connection of the prosthetic abutment) in correlation with the serum 25 (OH) vitamin D level. Moreover, the effect of vitamin D supplementation on osseointegration was assessed in human patients with end-stage renal disease (ESRD)  or vitamin D deficiency [38, 39] by the case reports.
Assessment of osseointegration
Kwiatek et al.  used conventional two-dimensional (2D) radiography to verify implant osseointegration and to assess changes in post-surgical crestal bone levels, respectively. In addition, they used ISQ (Implant stability quotient) measurement to assess the implant stability due to osseointegration. Serum vitamin D level have been measured in four studies [35, 36, 38, 39].
Two case reports [38, 39] with human subjects described those three patients have been losing their dental implant while having a vitamin D deficiency. Mangano et al.  discovered that patients with a low serum vitamin D level have a higher tendency to exhibit an EDIF.
Kwiatek et al.  showed in their study that vitamin D supplementation has an increasing influence on the change of bone level around dental implants. Fretwurst et al.  reported two cases in which vitamin D deficient patients have been successfully treated with dental implants after one or two consecutive dental implant failures, due to vitamin D supplementation. Flanagan et al.  reports one case in which a patient with ESRD was successfully treated with dental implants due to vitamin D supplementation and additional medication (Fig. 4).
From the literature reviewed, 18 studies [22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39] fulfilled our eligibility criteria; however, results from all animal studies [23, 31, 32] which compared vitamin D deficient to non-deficient subjects showed that BIC or new bone formation have been declined. According to the Institute of medicine (IOM) approximately 20–100% of the adult women and men living in northern Europe and North America appear to have vitamin D deficiency [5, 40,41,42,43,44]. Therefore, this finding seems to have a high clinical relevance on dental treatment in these regions.
The result from 75% [22,23,24,25, 32, 34] of the studies showed that systemically vitamin D supplementation enhanced new bone formation around dental implants. However, Pimentel et al.  and Akhavan et al.  showed no significant difference of new bone formation or bone to implant contact in subjects with systematically vitamin D supplementation. In fact, the study of Pimentel et al.  has been the only study with healthy systematically vitamin D supplemented animals. The supplementation of healthy subjects with vitamin D has only a weak improving effect. Vitamin D sufficient or insufficient patients benefit less of a vitamin D supplementation than deficient patients . In healthy patients a high-dose vitamin D supplementation can cause a lower bone mineral density .
Two studies [22, 30] have assessed the effect of vitamin D supplementation on osseointegration of diabetic rats. Both couldn’t show a statistically significant difference between vitamin D supplemented and not supplemented rats regarding BIC and new bone formation. The combined treatment of insulin and vitamin D supplementation  led to a significant higher BIC and BA compared to not treated diabetic rats and similar results as rats in the healthy control group. However, the supplementation of vitamin D in pre-diabetic, vitamin D deficient patients could reduce the risk of developing type 2 diabetes by 62% .
The period of vitamin D supplementation in the animal studies ranged between 7 day post-surgery and 8 week pre-surgery and 4 week post-surgery. Most studies [22, 25, 30, 33, 34] started post-surgery with the vitamin D supplementation. Only three studies [20, 21, 31] started pre-surgery. Dvorak et al.  and Nakamura et al.  assessed the effect of different supplementation periods on the osseointegration. Dvorak et al.  discovered that the deficient group had significantly less cortical BIC compared to the group which was supplemented 8 week pre-surgery and 14 day post-surgery. A different group which was supplemented 14 day pre-surgery and post-surgery had no significant change in BIC; however, the BV/TV was significantly decreased compared to the two other groups. Nakamura et al.  could not show a statistically significant difference between pre-surgery or post-surgery supplemented, ovariectomized rats. Neither in animals nor in humans there is a common recommendation on how and when to supplement or measure the 25 (OH) vitamin D level [46, 47].
Analog to animals, humans also showed a negative effect of low vitamin D levels on the osseointegration of dental implants. Mangano et al.  discovered that the number of EDIF’s has a tendency to increase when the patients show lower vitamin D levels. This finding leads to the hypothesis that humans similar to animals may also have an inferior osseointegration of implants due to vitamin D deficiency compared with subjects with an adequate vitamin D level. Furthermore, Kwiatek et al.  showed that systemically vitamin D supplementation enhanced new bone formation around dental implants. This result is similar to most of the studies carried out with animals and is slight evidence for a commonality with animals in this feature. All case reports [37,38,39] support the assumption that human beings have comparable symptoms when vitamin D deficient, supplementation of vitamin D seems to reduce these symptoms and help patients with a disturbed mineral homeostasis.
Only 50% of the animal studies [26, 27] showed that local vitamin D supplementation enhanced the bone to implant contact. The other two studies [28, 29] did not show significant difference in bone to implant contact, though on a cellular level there was a certain difference. Satué et al.  reported a significantly higher level of LHD around implants coated with a high dose of UV-irradiated vitamin D precursor and vitamin E compared to control. However, the low dosed coated implants had a significantly higher level of ALP (Alkaline phosphatase) compared with the high dosed coated implants. The osteocalcin level was significantly higher in the group of low doses coated implants. These results lead to the hypothesis that different concentrations of implant coatings have oppositional effects on the surrounding bone tissue.
It is relevant to mention that the majority of the studies [22,23,24,25,26,27,28,29,30,31,32,33,34] which assessed the effect of vitamin D deficiency or supplementation on osteogenesis around implants were performed in animals and the used methods remarkably varied among the studies included. The subjects in which the osseointegration around implants were assessed were mice, rats, rabbits and dogs. Some studies relied on histological analysis [22,23,24,25,26,27, 29, 30, 32] to evaluate new bone formation, others based on micro-CT analysis [22, 25, 33], while different depend on SEM analysis [31, 34]. Micro-CTs have higher resolution than computed tomography used for patients and are able to measure the bone mineral density. Cancellous bone can be evaluated by means of the trabeculae characteristics and their connectivity . This enables a far more distinct assessment of the bone and bone–implant interface. Histological analysis continues to be the gold standard for assessing bone formation on a cellular basis; however, they both provided comparable results for BIC, BA and bone–implant volume [49, 50].
Due to the very short follow-up period of the animal studies (up to 12 weeks) only little is known about the long-term effects of vitamin D supplementation on the survival rate of implants. Further studies are necessary to evaluate these long-term effects on the implant survival and may provide stronger evidence for the supporting effect of vitamin D on osseointegration. Animals with systemic diseases such as diabetes, osteoporosis and CKD did have a great benefit of BIC and/or new bone formation after a combined treatment with their standard medication plus vitamin D supplementation. Longitudinal, randomized controlled trials with human beings are needed to test whether this positive effect of vitamin D supplementation also applies on human beings and, therefore, improve the implant treatment of patients with these diseases.
Since there was just a small number of studies [35, 36] their results imply only weak evidence of the improvement on osseointegration due to vitamin D supplementation in humans. The case reports [37,38,39] investigating the effect of vitamin D on osseointegration in humans have a high risk of bias. Especially the effect of vitamin D supplementation on osseointegration in patients with diseases such as diabetes, osteoporosis, vitamin D deficiency and kidney diseases remains widely unknown.
Within the limitation of the present systematic review, it is concluded that vitamin D deficiency seems to have a negative effect on the osseointegration of implants in animals. The supplementation of vitamin D appears to enhance the osseointegration in animals with systemic diseases, such as vitamin D deficiency, diabetes mellitus, osteoporosis and CKD. The effect of vitamin D coated implants on the osseointegration remains controversial. Only slight evidence supports the hypothesis that humans similarly benefit from vitamin D supplementation in terms of osseointegration. Further investigations on the effect of vitamin D on osseointegration of dental implants in humans are required to maintain these assumptions.
Availability of data and materials
The included studies are available within PubMed/Medline (National library of Medicine, Washington, DC, USA), Cochane library and Google-Scholar databases.
Bone area density
Bone to implant contact
Bone volume/tissue volume
Chronic kidney disease
Demineralized allogeneic bone matrix
Early dental implant failures
End-stage renal disease
Institute of medicine
Implant stability quotient
Joscha G. Werny
Microscopic, computerized tomography
New bone formation
Participants, intervention, control, outcomes
Preferred reporting items for systematic reviews and meta-analysis
Scanning electron microscope
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J. G. Werny, K. Sagheb, L. Díaz Agurto, P. W. Kämmerer, B. Al-Nawas and E. Schiegnitz declare no conflicts of interest with respect to the research, authorship, and/or publication of this article. J. G. Werny, K. Sagheb, L. Díaz Agurto, P. W. Kämmerer, B. Al-Nawas and E. Schiegnitz do not have any financial interests, either directly or indirectly, related to the products or information discussed in the paper.
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Werny, J.G., Sagheb, K., Diaz, L. et al. Does vitamin D have an effect on osseointegration of dental implants? A systematic review. Int J Implant Dent 8, 16 (2022). https://doi.org/10.1186/s40729-022-00414-6