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International Journal of Implant Dentistry
Fig. 5 | International Journal of Implant Dentistry

Fig. 5

From: Characterization of bioactive substances involved in the induction of bone augmentation using demineralized bone sheets

Fig. 5

In vitro binding study of CF-hTGF-β1 with CF-hDMP1, CF-hMEPE, and CF-hBGN. A IE-HPLC chromatograms showing absorbance at 220 nm. B ALP-inducing activity of HPDL cells exposed to fractions 1 to 13 in each sample. CF-hTGF-β1 without incubation for the binding experiment (1 ng/mL) was used as a positive control. Data are means ± SEs of 3 culture wells (*p < 0.05, Mann–Whitney U test). C SDS–PAGE (5–20% gradient gel) stained with silver showing CF-hDMP1 (top), CF-hMEPE (middle), and CF-hBGN (bottom) after binding to CF-hTGF-β1 in fractions 1–13

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