Fig. 1

Preparation of demineralized bone sheets and animal transplantation experiments. A Flow chart showing the procedures used to generate four types of demineralized bone sheets. Sup: supernatant, Ppt: precipitate. B Photograph of a representative demineralized bone sheet. Each demineralized bone sheet was trimmed to approximately 3.0 mm (width) × 3.0 mm (depth) × 0.3 mm (height). C Schematic diagram showing the procedures for the animal transplantation experiment. The demineralized bone sheets, DBS-P, DBS-E, DBS-R and DBS-C, were transplanted subcutaneously under the axilla of 6-week-old male SD rats. The rats were sacrificed 8 weeks later, and the demineralized bone sheets were recovered. Each demineralized bone sheet was analysed with a micro-CT system