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International Journal of Implant Dentistry
Fig. 5 | International Journal of Implant Dentistry

Fig. 5

From: The extract of concentrated growth factor enhances osteogenic activity of osteoblast through PI3K/AKT pathway and promotes bone regeneration in vivo

Fig. 5

TRAP/ALP staining analysis. The images were photographed under a light microscope at ×40 and ×200 magnifications. (Black asterisk, new bone tissue; black arrows, TRAP positive osteoclasts which were stained purple; green arrows, ALP-positive osteoblasts which were stained brown) For quantification of TRAP staining, the mean of number of positive cells was determined from three serial sections; for quantification of ALP staining, the mean density (MD) was measured. At 2 weeks, the osteoclast number and the MD of ALP in group BC were significantly higher than that of groups C and B; at 6 weeks, the osteoclast number of group BC was obviously lower than that of groups C and B; conversely, the mean density (MD) of ALP in group BC was significantly higher than C and B groups. *p < 0.05; **p < 0.01

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