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International Journal of Implant Dentistry
Fig. 2 | International Journal of Implant Dentistry

Fig. 2

From: The extract of concentrated growth factor enhances osteogenic activity of osteoblast through PI3K/AKT pathway and promotes bone regeneration in vivo

Fig. 2

Effects of CGF-e on osteoblast proliferation and differentiation. a Proliferation analysis of MC3T3-E1 cells, the optical density value of CGF-e group was significantly higher than control group at 3 days. b ALP staining and enzyme activity of MC3T3-E1 cells, the CGF-e group exhibited more ALP-stained spot than control. Pictures of the ALP staining plates were taken using a Canon 60d camera (no magnification). Similarly, the enzyme activity in the CGF-e group was significantly higher than control at 3 days. c Relative gene expression of MC3T3-E1 cells, the relative gene expression of the two genes was significantly higher in the CGF-e group compared with the control group (BMP-2, bone morphogenetic protein-2; OCN, osteocalcin; PI3K, phosphoinositide 3-kinase). d Mineralization analysis of MC3T3-E1 cells, the number of calcium nodules of the CGF-e group was more than the control group. Pictures of the Alizarin Red staining plates were taken using a Canon 60d camera (no magnification); the samples were then examined under a light microscope at ×40 magnification. Quantitative analyses showed that absorbance value of CGF-e group was higher than control. *p < 0.05; **p < 0.01

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