Skip to main content

Table 1 The primers for qRT-PCR

From: Facile distribution of an alkaline microenvironment improves human bone marrow mesenchymal stem cell osteogenesis on a titanium surface through the ITG/FAK/ALP pathway

Genes Forward sequences Reverse sequences
GAPDH 5′-AACTTTGGCATTGTGGAAGG-3′ 5′-ACACATTGGGGGTAGGAACA-3′
Itg α2 5′-TTAGCGCTCAGTCAAGGCAT-3′ 5′-CGGTTCTCAGGAAAGCCACT-3′
Itg αV 5′-TTGTTTCAGGAGTTCCAAGA-3′ 5′-TGAAGAGAGGTGCTCCAATA-3′
Itg β1 5′-GCAGGCGTGGTTGCCGGAAT-3′ 5′-TTTTCACCCGTGTCCCACTTGGC-3′
Col1-α1 5′-CCAACGAGATCGAGCTCAGG-3′ 5′-GACTGTCTTGCCCCAAGTTCC-3′
Runx2 5′-TCTTCCCAAAGCCAGAGCG-3′ 5′-TGCCATTCGAGGTGGTCG-3′
ALP 5′-ACTCTCCGAGATGGTGGTGG-3′ 5′- CGTGGTCAATTCTGCCTCCTT-3′
OPG 5′-TAACGTGATGAGCGTACGGG-3′ 5′-GCAGCACAGCAACTTGTTCA-3′
RankL 5′-GAACACGCGTATTTACCTGC-3′ 5′-CCCATAGCCCACATGCAGTT-3
TGF-β1 5′-CGACTCGCCAGAGTGGTTAT-3′ 5′-AGTGAACCCGTTGATGTCCA-3′
BMP-2 5′-TCAAGCCAAACACAAACAGC-3′ 5′-AGCCACAATCCAGTCATTCC-3′
OSX 5′-CCTCAGGCCACCCGTTG-3′ 5′-AGCCATAGTGAACTTCCTCCTC-3′
OCN 5′-CAGTAAGGTGGTGAATAGAC-3′ 5′-GGTGCCATAGATGCGCTTG-3′