Fig. 5
From: The effect of nano-hydroxyapatite/chitosan scaffolds on rat calvarial defects for bone regeneration
Using a digital drawing and crop tool the regions of interest (ROIs) were segmented relative to the adjacent structures (a). The yellow line designates the lateral area inward (l1) or outward (l2) of middle sagittal seam of the newly formed bone and the green line designates the area of central bone formation (c), while the blue circular line specifies the area of the remained biomaterial (BmA). Τhe total number of osteocytes (Ost-arrows) in the newly formed bone surface was identified and used to estimate the osteocyte cell density (CD; Ost/mm2) in each ROI of both experimental and control groups (b) [hematoxylin and eosin stain, original magnifications for A × 20. Inset figure b was prepared using ObjectiveViewer (https://www.objectivepathology.com/objectiveview)]