Fig. 4
From: The effect of nano-hydroxyapatite/chitosan scaffolds on rat calvarial defects for bone regeneration
New bone formation (NBF) over time in both control and experimental groups; in rats euthanized at 2 weeks postoperatively (a–c), some NBF was observed on both experimental and control group, but not in the central area. At 4 weeks (d–f) and 8 weeks (g–i) postoperatively, a greater NBF area was observed on the inward (lateral 1; l1) or outward (lateral 2; l2) of middle sagittal seam lateral and in some samples also on the central region of the experimental group relative to the control group. The surface area of the biomaterial (BmA) decreased between the 2nd and 8th week post-surgery [hematoxylin and eosin stain, original magnifications for a, d and g × 20. Inset figures b, c, e, f, h, and i were prepared using ObjectiveViewer (https://www.objectivepathology.com/objectiveview)]