Skip to main content
International Journal of Implant Dentistry
Fig. 1 | International Journal of Implant Dentistry

Fig. 1

From: Aspergillus fumigatus biofilm formation on different bone substitutes used in maxillary sinus augmentation: an in vitro analysis

Fig. 1

CLSM and SEM micrographs of the A. fumigatus biofilm on five different bone substitutes. Xenograft: anorganic bovine bone; ß-TCP: ß-tricalcium phosphate; NHA: synthetic nano-hydroxyapatite; Allograft: demineralized bone matrix; HA: synthetic hydroxyapatite. 105 conidia/ml inoculated onto the different materials were incubated for 8 h to allow adhesion. After washing the unbound cells, the biofilm was grown in fresh medium for 20 h, washed gently with PBS and analyzed with CLSM (a, b, c) and SEM (d). a Orthogonal view, showing the mycelium morphology, characterized by highly intertwined hyphae. b 3D volume image, displaying the biofilm pellicle distributed over the surface of the materials. c 3D surface plot, revealing the lower density of the superficial layer of the biofilm formed on NHA and allograft. d × 600 magnification SEM micrographs: conidiophore detected in the biofilm formed on NHA and allograft are indicated by yellow arrows

Back to article page