Table 6 In vitro studies that investigated the effect of NSAIDs on osteoblasts attached to titanium surfaces
Study (year) | Sample | Treatment group | Methodology | Parameter | Outcome |
|---|---|---|---|---|---|
Arpornmaeklong et al. (2009) [19] | Mouse calvaria cell line (MC3T3-E1) | Indomethacin 0.1Ā Ī¼M Celecoxib 1.5Ā Ī¼M Celecoxib 3.0Ā Ī¼M Celecoxib 9.0Ā Ī¼M Control | Incubation in treatment medium for 5Ā days. Investigations were performed in three experimental phases: static, log, and plateau | The following parameters were assessed at 1, 3, and 5Ā days: cell attachment, cell growth, cell differentiation, secretion of PGE2 | Cells were able to grow and attach to titanium surface for all treatment groups. Indomethacin and celecoxib cell growth on days 3 and 5 in static phase and on day 3 in log phase. Indomethacin and celecoxib caused a significant decrease PGE2 concentration in static and plateau but not log phases. |
Boyan et al. (2001) [20] | Human osteosarcoma cell line (MG63) | Indomethacin 0.1Ā Ī¼M Resveratrol 1Ā Ī¼M Resveratrol 10Ā Ī¼M NS-398 1Ā Ī¼M NS-398 10Ā Ī¼M | Incubation in treatment medium for 5Ā days. Cells were cultured on tissue culture plastic, smooth titanium, and two rough titanium surfaces: grit-blasted/acid-etched and titanium-plasma sprayed | The following parameters were assessed after 5Ā days: osteocalcin content, PGE2 content, and TGF-Ī²1 content. | Indomethacin, resveratrol, and NS-398 had no effect on osteocalcin content. Indomethacin and resveratrol blocked PGE2 production. NS-398 had no effect on PGE2 production on smooth surfaces but caused a reduction on rough surfaces. Indomethacin blocked TGF-Ī²1 production on rough surfaces. Resveratrol blocked TGF-Ī²1 on TPS. NS-398 did not cause TGF-Ī²1 inhibition. |