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Table 6 In vitro studies that investigated the effect of NSAIDs on osteoblasts attached to titanium surfaces

From: The effect of non-steroidal anti-inflammatory drugs on the osteogenic activity in osseointegration: a systematic review

Study (year) Sample Treatment group Methodology Parameter Outcome
Arpornmaeklong et al. (2009) [19] Mouse calvaria cell line (MC3T3-E1) Indomethacin 0.1 μM
Celecoxib 1.5 μM
Celecoxib 3.0 μM
Celecoxib 9.0 μM
Incubation in treatment medium for 5 days.
Investigations were performed in three experimental phases: static, log, and plateau
The following parameters were assessed at 1, 3, and 5 days: cell attachment, cell growth, cell differentiation, secretion of PGE2 Cells were able to grow and attach to titanium surface for all treatment groups.
Indomethacin and celecoxib cell growth on days 3 and 5 in static phase and on day 3 in log phase.
Indomethacin and celecoxib caused a significant decrease PGE2 concentration in static and plateau but not log phases.
Boyan et al. (2001) [20] Human osteosarcoma cell line (MG63) Indomethacin 0.1 μM
Resveratrol 1 μM
Resveratrol 10 μM
NS-398 1 μM
NS-398 10 μM
Incubation in treatment medium for 5 days.
Cells were cultured on tissue culture plastic, smooth titanium, and two rough titanium surfaces: grit-blasted/acid-etched and titanium-plasma sprayed
The following parameters were assessed after 5 days: osteocalcin content, PGE2 content, and TGF-β1 content. Indomethacin, resveratrol, and NS-398 had no effect on osteocalcin content.
Indomethacin and resveratrol blocked PGE2 production. NS-398 had no effect on PGE2 production on smooth surfaces but caused a reduction on rough surfaces.
Indomethacin blocked TGF-β1 production on rough surfaces. Resveratrol blocked TGF-β1 on TPS. NS-398 did not cause TGF-β1 inhibition.