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Table 2 Listing of the culture media and additives together with manufacturers’ data

From: Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up

Culture medium/additives Manufacturer Order no. Concentration
Dulbecco’s modified Eagle medium (DMEM) with l-glutamine, plus 4.5 g glucose, without NaHCO3, without Natriumpyruvate Life Technology, Carlsbad, USA 11966-025  
Penicillin/streptomycin Life Technology, Carlsbad, USA 15640-055 100 IU penicillin/100 μg streptomycin/ml
Ca2+/Mg2+ free Hanks’ balanced salt solution (HBSS)–buffer Life Technology, Carlsbad, USA 14170-070  
Fetal calf serum (FCS) Life Technology, Carlsbad, USA 10270-098 10%
l-Glutamate Life Technology, Carlsbad, USA 25030-024 1%
Ethylenediaminetetraacetic acid (EDTA) solution Sigma Aldrich, St. Louis, USA E7889 0.5 M
l-Ascorbic acid 2-phosphate Sigma Aldrich, St. Louis, USA A-8960 1 mmol/l ASC (affinity isolated antibody)
Dexamethasone Serva Bioproducts, Heidelberg, Germany 18660 100 nmol/l