Table 2 Listing of the culture media and additives together with manufacturers’ data
From: Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up
Culture medium/additives | Manufacturer | Order no. | Concentration |
|---|---|---|---|
Dulbecco’s modified Eagle medium (DMEM) with l-glutamine, plus 4.5 g glucose, without NaHCO3, without Natriumpyruvate | Life Technology, Carlsbad, USA | 11966-025 | |
Penicillin/streptomycin | Life Technology, Carlsbad, USA | 15640-055 | 100 IU penicillin/100 μg streptomycin/ml |
Ca2+/Mg2+ free Hanks’ balanced salt solution (HBSS)–buffer | Life Technology, Carlsbad, USA | 14170-070 | |
Fetal calf serum (FCS) | Life Technology, Carlsbad, USA | 10270-098 | 10% |
l-Glutamate | Life Technology, Carlsbad, USA | 25030-024 | 1% |
Ethylenediaminetetraacetic acid (EDTA) solution | Sigma Aldrich, St. Louis, USA | E7889 | 0.5 M |
l-Ascorbic acid 2-phosphate | Sigma Aldrich, St. Louis, USA | A-8960 | 1 mmol/l ASC (affinity isolated antibody) |
Dexamethasone | Serva Bioproducts, Heidelberg, Germany | 18660 | 100 nmol/l |