Fig. 4

a C2C12 cells (control) and pBMP-2-transfected C2C12 cells were seeded on a 24-well plate (50,000 cell/well) and cultured in DMEM medium for 48 h. ALPL assay showing upregulated ALPL activity in the BMP-2-transfected C2C12 cells. b Cell extracts of C2C12 cells and pBMP-2-transfected cells were run on a 10% SDS-PAGE under non-denaturing conditions. The gel was then stained with NBT/BCIP solution (upper panel). Western bloting of actin showing the equal protein loading on the gel (lower panel). c Increased proliferation of C2C12 cells transfected with BMP-2 as well as ALPL activity when seeded on 3M™ESPE™ MDI disks. However, when the number of ALPL-positive cells is normalized to the total cell number, no differences are observed