Fig. 5

Histomicrograph of a biopsy from the BC + EMD group, showing an aspect of newly formed bone. Section stained with picrosirius-hematoxylin and digitalized with bright-field (d) and linearly polarized light (e and f). e, f Results of near 45° section rotation (between axes B–B’ and C–C’) to compensate some of the orientation-related effects associated with linearly polarized light. In d, typical Haversian systems are showed (area observed above dotted line, a to a’). In e and f, the arrows indicate thin birefringents (appears visually as brilliance) collagen arrangement around Haversian canals suggestive of lamellar mature bone. Areas observed below dotted lines are suggestive of immature (non-lamellar) bone where collagen fiber undulations can be observed. The dark area corresponds to complete disorganization of the collagen fibers. Bar = 100 μm