Table 1 Overview of bacterial species, corresponding genes, primers/probes, and DNA sequences
From: Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites
Species | Gene | Primer/probe | Sequences (5′-3′) |
|---|---|---|---|
Mycoplasma salivarium | rpoB | msali-F | CCG TCA AAT GAT TTC GAT TGC |
msali-R | GAA CTG CTT GAC GTT GCA TGT T | ||
msali-S | Hex-ATG ATG CTA ACC GTG CGC TTA TGG GTG-BHQ1 | ||
Veillonella parvula | 16S rDNA | vpar-F | TGC TAA TAC CGC ATA CGA TCT AAC C |
vpar-R | GCT TAT AAA TAG AGG CCA CCT TTC A | ||
vpar-S | HEX-CTA TCC TCG ATG CCG A-BHQ1 | ||
Staphylococcus aureus | nuc | saur-F | CAA AGC ATC CTA AAA AAG GTG TAG AGA |
saur-R | TTC AAT TTT CTT TGC ATT TTC TAC CA | ||
saur-S | FAM-TTT TCG TAA ATG CAC TTG CTT CAG GAC CA-BHQ1 | ||
Porphyromonas gingivalis | Arg-gingipain | pgin-F | CCT ACG TGT ACG GAC AGA GCT ATA |
pgin-R | AGG ATC GCT CAG CGT AGC ATT | ||
pgin-S | TexRed-TCG CCC GGG AAG AAC TTG TCT TCA-BHQ2 | ||
Parvimonas micra | 16S rDNA | pmic-F | TCG AAC GTG ATT TTT GTG GAA A |
pmic-R | GGT AGG TTG CTC ACG TGT TAC TCA | ||
pmic-S | FAM-CCC GTT CGC CAC TT-BHQ1 | ||
Tannerella forsythia | bspA | tfor-F | TCC CAA AGA CGC GGA TAT CA |
tfor-R | ACG GTC GCG ATG TCA TTG T | ||
tfor-S | FAM-CCG CGA CGT GAA ATG GTA TTC CTC-BHQ1 | ||
tfor-S II | HEX-TCG CGA CGT GAA ATG GTA TTC CTC-BHQ1 |